Electrochemical disinfection of fish pathogens in seawater without the production of a lethal concentration of chlorine using a flow reactor(ENVIRONMENTAL BIOTECHNOLOGY)
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概要
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An electrochemical disinfection system employing a honeycombed platinum coated titanium electrode was developed for the disinfection of seawater. Cell suspensions (2 1,10^3 cells/ml) of the fish pathogens, Vibrio alginolyticus, Edwardsiella tarda, Lactococcus garvieae and Vibrio anguillarum were circulated in a reactor equipped with 10 sets of these electrodes at a flow rate of 200 ml/min with an applied potential of 1.0 V vs. Ag/AgCl reference electrode. The circulated cells were completely disinfected after 3 h of treatment, whereas free residual chlorine generated due to seawater electrolysis was below 0.1 ppm. In addition, a diphenyl-1-pyrenylphosphine fluorescent assay revealed that lipid peroxidation in the cell membranes of disinfected bacteria was induced probably by reactive oxygen species generated during electrochemical treatment.
著者
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Matsunaga Tadashi
Division Of Biotechnology And Life Science Institute Of Engineering Tokyo University Of Agriculture And Technology
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Shionoiri Nozomi
Division Of Biotechnology And Life Science Institute Of Engineering Tokyo University Of Agriculture And Technology
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Tanaka Tsuyoshi
Division Of Biotechnology And Life Science Institute Of Engineering Tokyo University Of Agriculture And Technology
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Hosokawa Masahito
Division Of Biotechnology And Life Science Institute Of Engineering Tokyo University Of Agriculture And Technology
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Taguchi Tomoyuki
Yokogawa Electric Corporation
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WAKE Hitoshi
Yokogawa Electric Corporation
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Tanaka Tsuyoshi
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology
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HOSOKAWA Masahito
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology
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Shimoda Mari
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology
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