生薬「オウゴン」国内市場品の一酸化窒素産生抑制活性とLC/MSメタボローム解析

概要

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During the course of our studies on the profiling analysis for the crude drug, we collected various samples of scutellariae radix (Scutellaria baicalensis Georgi) from the Japanese market. This crude drug is one of the most widespread herbs used for Kampo medicine. The profiling data of the hot water extract of scutellariae radix were developed by LC/MS-based metabolomics. We examined its inhibitory activity on nitric oxide (NO) production by LPS/IFN-γ activated macrophages. The hot water extracts showed varied inhibitory activity against NO production from LPS/IFN-γ activated macropharges. Therefore, we performed PCA, OPLS and OPLS-DA with the SIMCA method to search for important markers which contributed antiinflammatory effect. As a result, the hot water extracts were clearly divided into two groups according to the strength of activity. We quickly found out that wogonoside, a main flavonoid, was a contributor to the distinction between the two groups by S plot. Wogonoside showed the inhibitory effect against NO production in a dose-dependent manner. These results suggested that wogonoside might be a useful biomarker to estimate the anti-inflammatory effect of scutellariae radix.
2013-08-20