Methyl and ethyl chloroformate derivatizations for compound-specific stable isotope analysis of fatty acids-II
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概要
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Methyl (MCF) and ethyl chloroformate (ECF) derivatizations are potentially useful for rapid and simple esterification (i.e., at room temperature for 5 min) prior to compound-specific stable isotope analysis (CSIA) of fatty acids. In this study, we have used acetonitrile as an organic solvent in the esterification process to improve the yields of derivatives, and we evaluated whether the improved esterification is suitable for carbon and hydrogen CSIA. Six saturated (14:0, 16:0, 18:0, 20:0, 22:0, and 24:0) and three unsaturated (18:1 n-9, 18:2 n-6, and 18:3 n-3) fatty acids were used in this study. The yields of derivatives are 67-107% for MCF and 84-89% for ECF derivatizations, which are approximately twice the yields obtained in MCF and ECF derivatizations that did not use acetonitrile. The accuracy of the CSIA is <±0.4‰ (1σ) for carbon and <± 11‰ (1σ) for hydrogen, with no significant differences in these uncertainties observed for saturated and unsaturated fatty acids. Thus, MCF and ECF derivatizations using acetonitrile are suitable for the CSIA of fatty acids.
- 日本有機地球化学会の論文
- 2012-12-28
著者
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Goto Akiko
Institute Of Geology And Geoinformation Aist
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Korenaga Takashi
Faculty Of Integrated Arts And Sciences University Of Tokushima
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Chikaraishi Yoshito
Institute For Research On Earth Evolution Japan Agency For Marine-earth Science And Technology
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Fujino Tatsuya
Department Of Chemistry Graduate School Of Science And Engineering Tokyo Metropolitan University
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Korenaga Takashi
Faculty of Risk and Crisis Management, Chiba Institute of Science
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Goto Akiko
Institute of Science and Engineering, Kanazawa University
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Aoyagi Kanji
Department of Chemistry, Tokyo Metropolitan University
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Yamanaka Atsuko
Department of Chemistry, Tokyo Metropolitan University
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Fujino Tatsuya
Department of Chemistry, Tokyo Metropolitan University
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