Compound-specific stable carbon isotope analysis of lignin phenols by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS)(<SPECIAL ISSUE>Advances in molecular and stable isotope studies among the organic geochemical and related commu
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概要
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This study demonstrates the stable carbon isotope analysis of individual lignin phenols based on off-line degradation and derivatization by tetramethylammonium hydroxide (TMAH) thermochemolysis, semi-purification by high-performance liquid chromatography (HPLC), compound-specific isotope analysis (CSIA) by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS), and isotope effect correction by isotope mass balance calculation. In GC/C/IRMS, we obtained the following optimal conditions to accurately and precisely determine the isotopic composition: combustion temperature of 1000℃, He carrier flow rate of 1.6mL min^<-1>, and injection amount of lignin phenols of 30-140ng. HPLC semi-purification using a normal-phase silica column with a column oven (at 30℃) can achieve baseline-separation of individual lignin phenols on the GC chromatogram without isotopic fractionation. Isotopic fractionation during TMAH derivatization can be corrected by the logarithmic correlation between the isotopic composition of the incorporated carbon during TMAH derivatization and the ratio of relative abundance between lignin phenols and TMAH reagents. By using this method, the carbon isotopic composition of individual lignin phenols can be determined within a standard deviation (1σ) of 0.2‰-0.9‰, making this method suitable for molecular isotope studies in the field of organic geochemistry.
- 2010-12-24
著者
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YAMAMOTO Shuichi
Department of Ophthalmology, Chiba University Hospital
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Chikaraishi Yoshito
Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology
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Sonoda Kazuhiko
Department of Environmental Engineering for symbiosis, Faculty of Engineering, Soka University
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Takahashi Masashiro
Department of Environmental Engineering for symbiosis, Faculty of Engineering, Soka University
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Sonoda Kazuhiko
Department Of Environmental Engineering For Symbiosis Faculty Of Engineering Soka University
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Takahashi Masashiro
Department Of Environmental Engineering For Symbiosis Faculty Of Engineering Soka University
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Chikaraishi Yoshito
Institute For Research On Earth Evolution Japan Agency For Marine-earth Science And Technology
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Yamamoto Shuichi
Department Of Environmental Engineering For Symbiosis Faculty Of Engineering Soka University
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Yamamoto Shuichi
Department Of Applied Chemistry And Chemical Engineering Yamaguchi University
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力石 嘉人
Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology
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