Effects of flow field on the metabolic characteristics of Streptomyces lincolnensis in the industrial fermentation of lincomycin(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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In this work, the flow field in the existing fermentor with radial-flow impellers (C1) was studied using the computational fluid dynamics (CFD) software package Fluent, then the fermentor with radial-axial flow impellers (C2) was constructed and was compared with the C1 fermentor by CFD and experimental research. The simulation results revealed that the flow field in C2 fermentor had characteristics such as higher turbulent kinetic energy, gas holdup and shear rates. Metabolic variables of Streptomyces lincolnensis in the two fermentors such as carbon and nitrogen source consumption rates, specific growth rates (μ), hyphae morphologies, and lincomycin productivities were further explored. The correlation analysis between the experimental measurements and the simulation results indicated that the hyphae clustering and dry cell weight (DCW) decreasing at production stage were eliminated in C2 fermentor, which had higher gas volumetric mass transfer coefficient (K_La), dissolved oxygen (DO) concentration and consumption rates of nutrient materials. When C2 was employed in the fermentor, the specific growth rate of S. lincolnensis at growth stage was higher, and the maintenance metabolism together with secondary metabolism at production stage was kept at higher levels. As a result, the yield of lincomycin was achieved 7039 μg ml^<-1> when the 60 m^3 industrial fermentor was equipped with C2, which was increased by 46% compared to that obtained in the C1 fermentor.
- 2013-01-00
著者
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Chu Ju
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Chu Ju
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Li Xiao
College Of Materials Science And Engineering Beijing University Of Chemical Technology
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Tan Ya-Li
College of Chemistry and Biology Science, China Three Gorges University
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Zhang Jiang
College of Chemistry and Biology Science, China Three Gorges University
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Li Zhi-Hong
Hubei Province Key Lab. of Yeast Function, Angel Yeast Co., Ltd
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Yu Xue-Feng
Hubei Province Key Lab. of Yeast Function, Angel Yeast Co., Ltd
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Xia Jian-Ye
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Ge You-Qun
Jiangxi GuoYao Pharmaceutical Limited Liability Company
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Li Xiao
College of Chemistry and Biology Science, China Three Gorges University:Hubei Province Key Lab. of Yeast Function, Angel Yeast Co., Ltd.
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XIA Jian-ye
State Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and Technology
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- Effects of flow field on the metabolic characteristics of Streptomyces lincolnensis in the industrial fermentation of lincomycin(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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- Effects of flow field on the metabolic characteristics of Streptomyces lincolnensis in the industrial fermentation of lincomycin