Real-Time Viable-Cell Mass Monitoring in High-Cell-Density Fed-Batch Glutathione Fermentation by Saccharomyces cerevisiae T65 in Industrial Complex Medium(BIOCHEMICAL ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
An on-line monitoring of viable-cell mass in high-cell-density fed-batch cultivations of Saccharomyces cerevisiae grown on an industrial complex medium was performed with an in situ capacitance probe fitted to a 50-l fermentor. Conventional off-line biomass determinations of several parameters, including dry cell weight(DCW), optical density at 600 nm wavelength(OD_<600>), packed mycelial volume(PMV) and number of colony forming units(CFU), were performed throughout the bioprocess and then compared with on-line viable-cell concentrations measured using a capacitance probe. Capacitance versus viable biomass and all off-line biomass assay values were compared during glutathione fermentation in industrial complex culture media. As a result, the relationship between the number of colony forming units and capacitance with a correlation coefficient(R) of 0.995 was achieved. Simultaneously, compared with those determined by at-line indirect estimation methods including oxygen uptake rate(OUR) and carbon dioxide evolution rate(CER), the specific growth rates estimated by on-line capacitance measurement could be more reliable during glutathione fermentation. Therefore, it is concluded that a capacitance probe is a practical tool for real-time viable biomass monitoring in high-cell-density fed-batch cultivation in a complex medium.
- 社団法人日本生物工学会の論文
- 2008-04-25
著者
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Guo Mei-jin
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Xiong Zhi-Qiang
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Guo Yuan-Xin
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Chu Ju
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Zhuang Ying-Ping
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Zhang Si-Liang
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Guo Yuan-xin
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Zhang Si-liang
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Xiong Zhi-qiang
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Chu Ju
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Zhuang Ying-ping
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
関連論文
- Real-Time Viable-Cell Mass Monitoring in High-Cell-Density Fed-Batch Glutathione Fermentation by Saccharomyces cerevisiae T65 in Industrial Complex Medium(BIOCHEMICAL ENGINEERING)
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- Effects of flow field on the metabolic characteristics of Streptomyces lincolnensis in the industrial fermentation of lincomycin(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Comparison of Power Number for Paddle-Type Impellers by Three Methods
- Effects of flow field on the metabolic characteristics of Streptomyces lincolnensis in the industrial fermentation of lincomycin