漢方生薬「川〓(Cnidii Rhizoma)」の抗アレルギー作用とその活性成分について

概要

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Background: Preparations containing the aromatic herb Cnidium officinale Makino (Cnidii Rhizoma), known as Senkyu in Japan, are widely used for gynecopathy and allergic skin diseases. However, the therapeutic effects on allergy have not been well examined. In this study, we investigated the anti-allergic activities of Cnidii Rhizoma in cultured Rat Basophilic Leukemia cells (RBL-2H3). Methods: We measured the mRNA expression of interleukin (IL)-4, IL-13, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in the cells by quantitative reverse transcription polymerase chain reaction (RT-PCR). The cells were treated with anti-IgE antibody in the presence of 70% methanol extract of Cnidii Rhizoma (70% MeOH ext.) and its fractions. Results and discussion: Three compounds, including cnidilide (1), were isolated from the 70% MeOH ext. Cnidilide (1) showed strong anti-allergic properties: at the concentration of 300 μM, it reduced IL-4 and IL-13 mRNA expression by 85% and 95%, respectively. Cnidilide (1) has been identified as the dominant aroma component of Cnidii Rhizoma. Herbalist tradition holds that strongly-pungent Cnidii Rhizoma is more medicative, and our findings partially support these descriptions of efficacy. Cnidii Rhizoma with a rich aroma is more therapeutic for skin allergies.
2013-02-20