30A-B-7 Chondrocyte-Patterning by Dielectrophoresis and Regeneration of Anisotropic Cartilaginous Tissue(Dielectrophoretic Approach to Biomedical Applications)

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Recently, there have been efforts aimed at the repair and regeneration of damaged articular cartilage using tissue engineering. In tissue engineering therapy, cells are cultured in vitro to create a three dimensional (3-D) tissue designed to replace the damaged cartilage. Although the tissue engineering is a useful approach to regenerate cartilage, the mechanical property of the regenerated cartilage is not sufficient to replace natural cartilage. The purpose of this study is to create mechanically anisotropic cartilage using 3-D cell pattering technology. We developed an anisotropic cartilaginous tissue by cell patterning within hydrogel slabs using dielectrophoretic (DEP) forces. Our data indicate that the embedded chondrocytes remained viable and reconstructed cartilaginous tissue along the patterned cell array. DEP cell patterning may become a useful approach for reconstructing anisotropic structure in cartilage regeneration.
2011-10-29