薄層クロマトグラフィーけい光デンシトメトリーによる生薬中のベルベリン成分の定量
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The micro-determination of berberine in thirty samples of crude drugs was performed by means of thin layer chromatography-fluorometric densitometry system. Almost the same procedure of JP 8 was used in preparation of berberine sample for TLC, which was carried out by using precoated plate of Merck of 20×20 cm and n-BuOH : AcOH : H_2O (7 : 1 : 2) for development. In order to determine berberine the fluorometric devise of densitometer of CS-900 (Shimazu Co.) with linear scanning method was successfully applied (TABLE I).
- 1976-12-20
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- 薄層クロマトグラフィーけい光デンシトメトリーによる生薬中のベルベリン成分の定量