97. チューリップ花弁からのチューリッポシドA変換酵素遺伝子の単離(口頭発表,植物化学調節学会第46回大会)

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Tuliposides are major secondary metabolites in tulip (Tulipa gesneriana). Their lactonized aglycons, tulipalins, function as defensive chemicals due to their biological activities. We recently found that tuliposide conversion to tulipalin occurs enzymatically in tulip tissues, and the enzyme, tuliposide-converting enzyme (TCE), has been purified from tulip bulbs. However, the results raised a possibility that the TCE that is expressed in tissues other than bulbs is different from bulb TCE. To investigate the functional diversity of tulip TCE, the enzyme was first purified from petals as a representative for tissues other than bulbs. The purified enzyme preferentially accepted tuliposides as substrates, with 6-tuliposide A the best substrate, which allowed naming the enzyme 'tuliposide A-converting enzyme (TCEA)', and exhibited characteristics similar to bulb enzyme. However, specific activity and molecular mass differed significantly between the petal and bulb enzymes. Following peptide sequencing, novel cDNA (TgTCEA) encoding petal TCEA was isolated, and the functional characterization of the recombinant enzyme confirmed the involvement of TgTCEA in 6-tuliposide A conversion to tulipalin A. TgTCEA was transcribed in all tulip tissues, but not in bulbs, indicating the presence of a bulb-specific TgTCEA. The enzyme, TgTCEA, was the first identified member of the lactoneforming carboxylesterases, specifically catalyzing intramolecular transesterification.
2011-10-03

97. チューリップ花弁からのチューリッポシドA変換酵素遺伝子の単離(口頭発表,植物化学調節学会第46回大会)

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