大型褐藻類Ascophyllum nodosumから精製された硫酸基含有多糖体アスコフィラン及びフコイダンの免疫賦活作用を介した抗腫瘍効果

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Ascophyllan and fucoidan, fucose-containing sulfated polysaccharides isolated from brown seaweed Ascophyllum nodosum, have various biological activities in vitro. Our previous studies have demonstrated that these polysaccharides induce tumor necrosis factor-α (TNF-α) and granulocyte-colony stimulating factor (G-CSF) secretion from murine macrophage cell line, RAW264.7 cells in a concentration-dependent manner. In this study, we investigated in vivo effects of ascophyllan and fucoidan in mouse model in addition to in vitro studies. In Sarcoma180 ascites tumor-bearing mouse model, the increases in the survival rate of tumor-bearing mouse treated with intraperitoneal (i.p.) administration of ascophyllan and fucoidan were observed as compared to the control mouse injected with saline alone. Since ascophyllan and fucoidan showed no significant cytotoxicity on Sarcoma 180 cells in vitro, these polysaccharides may suppress tumor growth through the activation of host immune system rather than a direct cytotoxic effect on tumor cells. In fact, i.p. injection of ascophyllan and fucoidan into mouse resulted in significant increase in the serum levels of TNF-α and G-CSF.
2011-08-01