赤潮原因プランクトン,Chattonella marina に存在する活性酸素消去物質に関する研究

概要

論文の詳細を見る
Chattonella marina (C. marina), a harmful red tide phytoplankton, has been known to produce reactive oxygen species (ROS) under the normal physiological conditions. In this study, we found that C. marina has potent radical scavengers against superoxide anion ( O2-) and hydrogen peroxide as its own cellular components. The radical scavenging activity of the aqueous extracts from C. marina was examined by several assay systems. The extracts showed the strong scavenging activity against O2- generated enzymatically by xanthine xidase/hypoxanthine system. Furthermore, O2- generated by C. marina and Heterosigma akashiwo were also effectively scavenged by the extracts in a concentration-dependent manner, and in the presence of 100 mg/ml of the extracts, O2- levels in these red tide flagellate cell suspensions became undetectable levels. Consistent with these results, the growth inhibition of Vibrio alginolyticus caused by C. marina or H. akashiwo was significantly suppressed by the extracts with similar extent of catalase and superoxide dismutase. The extracts showed partial scavenging activity against hydrogen peroxide at the concentration at which O2- was almost completely scavenged, suggesting that relatively O2- specific scavengers may exist in the extracts. Dialysis and gel-filtration experiments suggested that relatively low molecular weight compounds present in the extracts may be responsible for the radical scavenging activity.
長崎大学の論文