Complete detoxification of tris(1,3-dichloro-2-propyl) phosphate by mixed two bacteria, Sphingobium sp. strain TCM1 and Arthrobacter sp. strain PY1(ENVIRONMENTAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Tris(1,3-dichloro-2-propyl) phosphate (TDCPP), a flame retardant, is regarded as a potentially toxic and persistent environmental contaminant. We previously isolated a TDCPP-degrading bacterium, Sphingobium sp. strain TCM1, which, however, produced a toxic metabolite: 1,3-dichloro-2-propanol (1,3-DCP). This study was undertaken to develop a technique for complete TDCPP detoxification using strain TCM1 with a 1,3-DCP-degrading bacterium, Arthrobacter sp. strain PY1. For efficient detoxification, we designed a resting cell system and examined the effect of freezing and lyophilization treatments for preparation of their resting cells. Results show that treatments had no marked adverse effect on their activities. The TDCPP dephosphorylation by TCM1 resting cells was optimal at 30℃ and pH 8.5. Also, 1,3-DCP dehalogenation by strain PY1 resting cells was optimal at 35℃ and pH 9.5. Under those respective conditions, the activities were 2.48μmolh^<-1>・OD_<660>^<-1> for TDCPP and 0.95μmolh^<-1>・OD_<660>^<-1> for 1,3-DCP. Based on these results, we set the reaction temperature to 30℃ and pH to 9.0. Then we examined the detoxification of 50μM TDCPP using mixed resting cells at a final OD_<660> of 0.05 for strain TCM1 and 0.2 for strain PY1. In these conditions, TDCPP was eliminated after 1h, but some of the resulting 1,3-DCP remained at a constant level. The increase in strain PY1 cells to a final OD_<660> of 4.0 decreased the TDCPP dephosphorylation rate of strain TCM1 cells but achieved complete detoxification of TDCPP during 12h of reaction.
著者
-
Takahashi Shouji
Department of Environmental Systems Engineering, Nagaoka University of Technology
-
Kera Yoshio
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Kera Y
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Akatsu Kumiko
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Abe Katsumasa
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Takahashi Shouji
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Okada Shohei
Department Of Cell Chemistry Institute Of Molecular And Cell Biology Okayama University Medical Scho
-
Okada Shohei
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Obana Yuki
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
KERA Yoshio
Department of Civil and Environmental Engineering, Nagaoka University of Technology
関連論文
- Enrichment and Characterization of Chlorinated Organophosphate Ester-Degrading Mixed Bacterial Cultures(ENVIRONMENTAL BIOTECNOLOGY)
- Cloning and Expression of the Pyridoxal 5'-Phosphate-Dependent Aspartate Racemase Gene from the Bivalve Mollusk Scapharca broughtonii and Characterization of the Recombinant Enzyme
- Cloning and Expression in Escherichia coli of the D-Aspartate Oxidase Gene from the Yeast Cryptococcus humicola and Characterization of the Recombinant Enzyme
- Isolation of the Cryptococcus humicolus URA3 Gene Encoding Orotidine-5'-Phosphate Decarboxylase and Its Use as a Selective Marker for Transformation (GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Determination of N-methyl-D-aspartate in tissues of bivalves by high-performance liquid chromatography
- Geranylgeranylacetone Induces Apoptosis in HL-60 Cells
- Complete detoxification of tris(1,3-dichloro-2-propyl) phosphate by mixed two bacteria, Sphingobium sp. strain TCM1 and Arthrobacter sp. strain PY1(ENVIRONMENTAL BIOTECHNOLOGY)
- Complete detoxification of tris(2-chloroethyl) phosphate by two bacterial strains: Sphingobium sp. strain TCM1 and Xanthobacter autotrophicus strain GJ10
- Complete detoxification of tris(2-chloroethyl) phosphate by two bacterial strains : Sphingobium sp. strain TCM1 and Xanthobacter autotrophicus strain GJ10(ENVIRONMENTAL BIOTECHNOLOGY)
- Suppression of acetaldehyde accumulation by 4-methylpyrazole in alcohol-hypersensitive Japanese.
- Complete detoxification of tris(2-chloroethyl) phosphate by two bacterial strains : Sphingobium sp. strain TCM1 and Xanthobacter autotrophicus strain GJ10