Enrichment and Characterization of Chlorinated Organophosphate Ester-Degrading Mixed Bacterial Cultures(ENVIRONMENTAL BIOTECNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Chlorinated organophosphate ester (OPE)-degrading enrichment cultures were obtained using tris(2-chloroethyl) phosphate (TCEP) or tris(1,3-dichloro-2-propyl) phosphate (TDCPP) as the sole phosphorus source. In cultures with 46 environmental samples, significant TCEP and TDCPP degradation was observed in 10 and 3 cultures, respectively, and successive subcultivation markedly increased their degradation rates. 67E and 45D stable enrichment cultures obtained with TCEP and TDCPP, respectively, completely degraded 20μM of the respective compounds within 6 h and also the other, although the degradation rate of TCEP by 45D was relatively slow. We confirmed chloride ion generation on degradation in both cases and the generation of 2-chloroethanol (2-CE) and 1,3-dichloro-2-propanol (1,3-DCP) as metabolites of TCEP and TDCPP, respectively. 67E and 45D also showed dehalogenation ability toward 2-CE and 1,3-DCP, respectively. Addition of inorganic phosphate did not significantly influence their ability to degrade the chlorinated OPEs but markedly increased their dehalogenation ability, which was maximum at 0.2mM of inorganic phosphate and decreased at a higher concentration. Denaturing gradient gel electrophoresis analysis showed that dominant bacteria in 67E are related to Acidovorax spp. and Sphingomonas spp. and those in 45D are Acidovorax spp., Aquabacterium spp., and Sphingomonas spp. This analysis indicated the relationship of the Sphingomonas- and Acidovorax-related bacteria with the cleavage of the phosphoester bond and dehalogenation, respectively, in both cultures. This is the first report on bacterial enrichment cultures capable of degrading both TCEP and TDCPP.
- 2008-07-25
著者
-
Yamada Ryo-hei
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Takahashi Shouji
Department of Environmental Systems Engineering, Nagaoka University of Technology
-
Kawashima Koji
Department of Environmental Systems Engineering, Nagaoka University of Technology
-
Kawasaki Manami
Department of Environmental Systems Engineering, Nagaoka University of Technology
-
Kamito Jun
Department of Environmental Systems Engineering, Nagaoka University of Technology
-
Endo Yusuke
Department of Environmental Systems Engineering, Nagaoka University of Technology
-
Akatsu Kumiko
Department of Environmental Systems Engineering, Nagaoka University of Technology
-
Horino Sadatoshi
Department of Environmental Systems Engineering, Nagaoka University of Technology
-
Kera Yoshio
Department of Environmental Systems Engineering, Nagaoka University of Technology
-
Horino Sadatoshi
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Kera Yoshio
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Kamito Jun
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Endo Yusuke
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Kera Y
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Akatsu Kumiko
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Kawashima Koji
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Abe Katsumasa
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Kawasaki Manami
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
Takahashi Shouji
Department Of Environmental Systems Engineering Nagaoka University Of Technology
-
KERA Yoshio
Department of Civil and Environmental Engineering, Nagaoka University of Technology
関連論文
- Enrichment and Characterization of Chlorinated Organophosphate Ester-Degrading Mixed Bacterial Cultures(ENVIRONMENTAL BIOTECNOLOGY)
- Schnurri-2 regulates T_h2-dependent airway inflammation and airway hyperresponsiveness
- Cloning and Expression of the Pyridoxal 5'-Phosphate-Dependent Aspartate Racemase Gene from the Bivalve Mollusk Scapharca broughtonii and Characterization of the Recombinant Enzyme
- Cloning and Expression in Escherichia coli of the D-Aspartate Oxidase Gene from the Yeast Cryptococcus humicola and Characterization of the Recombinant Enzyme
- Isolation of the Cryptococcus humicolus URA3 Gene Encoding Orotidine-5'-Phosphate Decarboxylase and Its Use as a Selective Marker for Transformation (GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Determination of N-methyl-D-aspartate in tissues of bivalves by high-performance liquid chromatography
- Complete detoxification of tris(1,3-dichloro-2-propyl) phosphate by mixed two bacteria, Sphingobium sp. strain TCM1 and Arthrobacter sp. strain PY1(ENVIRONMENTAL BIOTECHNOLOGY)
- Complete detoxification of tris(2-chloroethyl) phosphate by two bacterial strains: Sphingobium sp. strain TCM1 and Xanthobacter autotrophicus strain GJ10
- Complete detoxification of tris(2-chloroethyl) phosphate by two bacterial strains : Sphingobium sp. strain TCM1 and Xanthobacter autotrophicus strain GJ10(ENVIRONMENTAL BIOTECHNOLOGY)
- Suppression of acetaldehyde accumulation by 4-methylpyrazole in alcohol-hypersensitive Japanese.
- Complete detoxification of tris(2-chloroethyl) phosphate by two bacterial strains : Sphingobium sp. strain TCM1 and Xanthobacter autotrophicus strain GJ10
- S16-2 Pathogenic memory Th2 cells in the airway and regulation by activated NKT cells in vivo(Allergy and Innate Immunity (English Session))