薬用ニンジン形質転換根の増殖とジンセノサイド生産に与える植物ホルモンの影響

概要

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We investigated the effects of several phytohormones on the growth and ginsenosides contents of Panax ginseng strain R52, a strain having a rapidly growing root and obtained by transformation by Agrobacterium rhizogenes. Gibberellin A3 had little effect on the growth index and decreased the ginsenoside contents, whereas 3-indolebutyric acid decreased both the growth and its ginsenoside contents compared with those of the plant cultured without phytohormone. Benzyladenine and kinetin, each stimulated the growth of R52 with a slight decrease of the protopanaxatriol content. Benzyladenine promoted the growth by as much as 1.8-fold (or 30-fold/30 days of culture), and increased the ginsenoside contents by 1.5-fold those of the control cultured without hormone. This growth rate is the highest growth rate of ginseng tissue in batch culture so far reported.
日本生薬学会の論文
1995-09-20