微小透析法によるイチョウ緑葉エキスの末梢自律神経に及ぼす影響

概要

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The effects of a green leaf extract from Ginkgo biloba L. on the autonomic nervous system were examined by using in vivo microdialysis and high performance liquid chromatography equipped with an electrochemical detector to measure the extracellular levels of catecholamines in rats. The animals were anesthetized with sodium pentobarbital (50 mg/kg, i.p.) and the thigh was shaved. The microdialysis probe was inserted intracutaneously into the rat thigh through the insertion hole made by a 19G needle. During the infusion of Ringer's solution at a rate of 3μl/min, the extracellular levels of 3-methoxy-4-hydroxyphenyl glycol (MHPG) were monitored (20-min collection period). KCl added to the perfusion fluid at a concentration of 100 mM increased the extracellular MHPG levels to about 1.9-fold, whereas tetrodotoxin (50μm) added to the perfusion fluid, reduced the extracellular MHPG levels. These findings indicate that the extracellular MHPG levels recorded by this method are derived predominantly from peripheral neuronal sources. A green leaf extract from Ginkgo biloba L. added to the perfusion fluid increased the extracellular MHPG levels to 1.7 times the control level (p<0.05). The present findings suggest that releases of neurotransmitter from peripheral nerve endings may be stimulated enhanced by green leaf extract from Ginkgo biloba L.
日本生薬学会の論文
1995-06-20