J0202-2-2 厚みを持つ組織の凍結保存における解凍後生存率の改善に関する検討([J0202-2]バイオ熱・物質移動(2))

概要

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In cryopreservation of tissues or organs, the decision of the optimal freezing condition is difficult because the cell density of the frozen sample, the orientation of the cell, and the form influence cell viability after freezing. Until now, we have found the relative viability decreases by leaving from the cooling area (sample's bottom) with the result of having examined cell viability of human dermal fibroblast monolayer, which imitates the biological tissue, given the freezing direction. In this study, to find the condition to improve the viability decreasing, we examined the concentrated solution and the freezing curve at each part of sample and relationship with the cell viability after cryopreservation. The results showed the possibility that the concentrate of the cryoprotectant solution did not relate to the decrease of viability, and the difference of the cooling rate and the freezing curve in each part of a sample and the change of ice crystal formation with these differences relate to the viability decrease.
2010-09-04