S0201-2-4 ヒアルロン酸添加による凍結中の高密度単層細胞における細胞損傷の低減(マイクロ・ナノバイオメカニクス:細胞生物学への接近(2)刺激と応答)

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During slow freezing of the cells, mechanical stress with the contact of the cells is caused by extracellular freezing. The mechanical stress influences the decrease of post-thaw cell viability. In biological tissues, the cells come in direct contact mutually, and it is thought that the influence of mechanical stress is large. This study suggested the reduction of the cell damage by addition of hyaluronic acid (HA), which is in the body and has low cytotoxity, to cryoprotectant in cultured monolayer cells in high cell density during slow freezing. Cell viability in each condition of cultured monolayer cells of human dermal fibroblast frozen by only 10% dimethylsulfoxide (DMSO) or 10%DMSO added various concentration of HA was examined by counting living/dead cells from fluorescence images.
2009-09-12