B101 凍結保存された単層細胞において解凍後の生存率に及ぼす基質の影響(B1-1 細胞工学・マイクロバイオメカニクス1)

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To investigate the effects of culture substrate materials and morphology on post-thaw cell viability in cryopreservation, we cultured a human skin fibroblast on the glass and the polymer substrate fabricated with grooves processing, and evaluated the cell viability after cryopreservation. Monolayer cells on each substrate at the cell density of 5104cells/cm2 were frozen at a cooling rate of 0.1, 0.3, 1.0 3.0℃/min in a solution of Dulbecco's Modified Eagle Medium, 20% Fetal Bovine Serum and 10% dimethylsulfoxide. Relative viability of monolayer cells using fluorescence (Calcein-AM and DAPI) was evaluated. Results showed that the relative viability of monolayer on polymer substrate was higher than that of glass at 0.3 and 1.0℃/min. In addition, the difference in the relative viability was not seen, whether substrate with grooves or without. Therefore, we found that the polymer substrate was more suitable than glass as a culture substrate of the cell for cryopreservation.
2009-11-06