PEGylated antibody in organic media(BIOCHEMICAL ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
Antibodies were covalently conjugated with poly (ethylene glycol) (PEG) and the properties of the PEGylated antibodies in organic media were investigated. Two types of monoclonal antibody were used in this study. One was a monoclonal antibody (abzyme) that was prepared against a hapten mimicking a transition state of hydrolysis. Another was a monoclonal antibody against estrogen, which is not soluble in water. By electrophoresis and mass spectral analysis, the covalent conjugation with PEG chains was confirmed. The PEGylated antibodies bound to antigens and the PEGylated abzyme catalyzed a hydrolysis reaction in an aqueous solution. The PEGylated antibodies were soluble in dichloromethane and acetone and interacted with antigen either in dichloromethane or in acetone. In conclusion, PEGylated antibodies can be employed as analytical tools for water-insoluble analytes.
著者
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Fujii Ikuo
Department Of Biological Science Graduate School Of Science Osaka Prefecture University
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Liu Mingzhe
Nano Medical Engineering Laboratory Riken
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Tsumuraya Takeshi
Department Of Biological Science Graduate School Of Science Osaka Prefecture University
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ABE Hiroshi
Nano Medical Engineering Laboratory, RIKEN
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ITO Yoshihiro
Nano Medical Engineering Laboratory, RIKEN
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Son Tae
Nano Medical Engineering Laboratory Riken Advanced Science Institute:department Of Bioscience &
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Xu Muye
Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute
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Loh Xian
Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute
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Li Jun
Division of Bioengineering, Faculty of Engineering, National University of Singapore
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Loh Xian
Nano Medical Engineering Laboratory Riken Advanced Science Institute:division Of Bioengineering Facu
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Ito Yoshihiro
Nano Medical Engineering Laboratory Riken Advanced Science Institute
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Xu Muye
Nano Medical Engineering Laboratory Riken Advanced Science Institute
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Li Jun
Division Of Bioengineering Faculty Of Engineering National University Of Singapore
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Loh Xian
Nano Medical Engineering Laboratory Riken Advanced Science Institute
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Liu Mingzhe
Nano Medical Engineering Laboratory Riken Advanced Science Institute
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Son Tae
Nano Medical Engineering Laboratory Riken Advanced Science Institute
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Abe Hiroshi
Nano Medical Engineering Laboratory Riken Advanced Science Institute
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ITO Yoshihiro
Nano Medical Engineering Laboratory
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Tsumuraya Takeshi
Department of Biological Science, Graduate School of Science, Osaka Prefecture University
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