P-118 リグノセルロース糖化並行発酵細菌の代謝工学的育種(ポスター2,ポスター発表)

概要

論文の詳細を見る
In recent years it has been suggested that, instead of traditional feedstocks (starch crops), cellulosic biomass (cellulose and hemicellulose), including agricultural and forestry residues, waste paper, and industrial wastes, could be used as an ideally inexpensive and abundantly available source of sugar for fermentation into transportation fuel ethanol. We have been focusing on a useful new ethanol-fermenting bacterium, Zymobacter palmae, given its broad range carbohydrate substrates and ability to efficiently produce ethanol, but this organism could not ferment cellulose or its degradation products, cellooligosaccharides and cellobiose. Therefore, as the first step in breeding a strain of Zb. palmae that can produce ethanol from cellulosic materials directly, we investigated the possibility of conferring the ability to ferment cellobiose to the strain through metabolic engineering. To confer the ability to ferment cellobiose, which is a hydrolysis product from cellulosic materials treated enzymatically or with acid, the β-glucosidase gene from Ruminococcus albus was introduced into Zb. palmae, where its expression was driven by its endogenous promoter. About 56% of the enzyme expressed was localized on the cell-surface or in the periplasm. The recombinant Zb. palmae could ferment 2% cellobiose to ethanol, producing 95% of the theoretical yield with no accumulation of organic acids as metabolic by-products. Thus, expression of β-glucosidase in Zb. palmae expanded the substrate spectrum of the strain, enabling ethanol production from cellulosic materials.
2010-01-20

P-118 リグノセルロース糖化並行発酵細菌の代謝工学的育種(ポスター2,ポスター発表)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク