凍結保存Allo血管移植に対する免疫寛容の導入

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Calcification and aneurysma changes in cryopreserved aortic allografts result from cryopreservation, thawing and chronic immunological rejection. The influence of donor specific tolerance using donor bone marrow cells in cryopreserved aortic allograft transplantation was studied. Thoracic aorta harvested from Brown Norway rats was transplanted in the infra-renal position immediately (group F, n=5) or after cryopreservation (group C, n=6) in Lewis rats. Immuno-specific tolerance was induced by intrathymic inoculation of donor bone marrow cells harvested from a long bone and intraperitoneal administration of anti-rat lymphocyte serum (group I, n=5). The transplanted grafts were examined at 6 weeks after transplantation morphologically, pathologically and iounoouobiutochemically. Group C showed significant stenosis in morphological analysis. There was no significant difference in medial area ratio in each group, but intimal/medial area ratio of group C was significantly higher than that of other groups; therefore, intimal thickening with proliferation resulted in aortic allograft stenosis. Suppression of intimal thickening was confirmed in group I. In pathological examination, rejection of media was significantly greater in group C than in the other groups; suppression of rejection was observed in group I. Intimal injury and dehydration of adventitial cells were demonstrated in electron scanning microgram. Donor antigen-positive cells were detected in thymus of recipient rats by immunohistochemical staining, which confirmed microchimerism. In conclusion, induction of tolerance was effective in suppression of rejection of cryopreserved aortic allografts.
東京女子医科大学の論文
2000-12-25

凍結保存Allo血管移植に対する免疫寛容の導入

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