TGF-β1遺伝子の新しいスプライシングバリアントの同定と解析

概要

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Transforming growth factor-β1 (TGF-β1) is a multifunctional factor that has profound regulatory effects on many physiological processes. We investigated the role of TGF-β1 in cardiac myocyte differentiation using an embryonic carcinoma cell line, P19 cells, which can differentiate into cardiac myocytes under treatment with retinoic acid. In the process of this study, we identified a new splicing variant of the TGF-β1 genes. Expression of this variant, which we called "TGF-β1-SH", was enhanced through P19 cell differentiation. Reverse transcriptase-polymerase chain reaction and RNase protection assays were performed to examine TGF-β1-SH gene expression. The gene was expressed by 11.5 days post-coitum (dpc) mouse embryo and in various adult mouse tissues, especially in the spleen and heart. Sequence analysis revealed that this cDNA encodes a different carboxy-terminal structure protein from that expressed by the known TGF-β1 gene. Interestingly, treatment of cultured adult mouse cardiac fibroblasts with aldosterone decreased the expression of this variant. This result suggests that this TGF-β1-SH may play an important role in cardiac differentiation and also in the regulation of cardiac fibrosis.
東京女子医科大学の論文
2001-04-25