心筋の組織再生

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Recent progress in stem cell biology has encouraged current research on cardiac cell transplantation. Furthermore, "Cardiac Tissue Engineering", which means bioengineering of viable three-dimensional (3-D) heart tissue from cultured cardiomyocytes, has begun. Tissue engineering frequently exploits 3-D biodegradable scaffolds as a temporary extracellular matrix. This concept has also been applied to cardiac tissue engineering. In contrast to these technology, we have exploited the capabilities of novel cell culture surfaces grafted with a temperature-responsive polymer, poly (N-isopropylacrylamide) (PIPAAm), to produce intact viable monolayer cell sheets simply by reducing temperature and to construct 3-D tissues by layering cell sheets. Cultured cardiomyocyte sheets detached from PIPAAm-grafted surfaces were layered to construct cardiac grafts. In vitro study, the electrical communication was established between the sheets and the engineered constructs pulsed spontaneously and synchronously in macroscopic view. In vivo study, layered cardiomyocyte sheets were transplanted in subcutaneous tissues of nude rats. Three weeks after the transplantation, electrocardiograms originated from transplanted grafts were detected. When transplantation sites were opened, spontaneous contraction of the grafts were observed macroscopically. Histological studies showed characteristic heart tissue and sufficient blood vessels within the contractile tissues. Cardiac tissue engineering based on this technology "Cell Sheet Engineering" may prove useful for cardiovascular tissue repair.
東京女子医科大学の論文
2002-06-25

心筋の組織再生

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