PROBLEMS ON THE QUANTITATIVE ESTIMATION OF IMMUNOGLOBULINS BY SINGLE RADIAL IMMUNODIFFUSION METHOD
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概要
- 論文の詳細を見る
The accuracy of immunoglobulin values estimated by commercially available kits of single radial immunodiffusion method was examined. The results showed that the values were not necessarily the same amoung the kits from three companies. The maximum deviations between the indications of three standard sera were 17% with mg/100ml indication and 15% with IU/ml indication, and when unknown samples (control serum or patient sera) were estimated by using these kits, the maximum deviation reached more than 30% with mg/100ml indication. The main cause of these deviations appears to be attributable to the incorrect indications of the standard sera and partly to the different antibody concentrations in the immunodiffusion plate. A comparison between Mancini's and Fahey's methods showed that the deviations with Mancini's method were in most cases parallel to the deviation of values of the standard sera, while those with Fahey's procedure have no relationship to the deviations of the standard sera, suggesting that for accurate estimations Mancini's method is more suitable.
- 名古屋市立大学の論文
- 1978-08-28
著者
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佐々木 實
Department Of Biochemistry Nagoya City University Medical School
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松葉 和久
Department Of Biochemistry Nagoya City University Medical School
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佐々木 實
Department Of Acoustic Design Kyushu Institute Of Design
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佐々木 實
Department of Biochemistry, Nagoya City University Medical School
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