51.cDNAサブトラクション法による光学活性なウレア系化合物処理におけるコムギ根端発現遺伝子の探索(口頭発表)

概要

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The optical active urea compounds R-/S-1-α-methylbenzyl-3-p-tolylurea (R-/S-MBTU) shows very interesting property, S-MBTU inhibits root growth of rice companion weeds (Echinochloa species), but not that of rice. Whereas, R-MBTU inhibits root growth of wheat companion weeds (Avena, Alopecurus and Beckmannia species), but not that of wheat. Thus, R-/S-MBTU shows enantioselective herbicidal activity, but there is little information on the biochemical and molecular biological aspects for R-/S-MBTU mode of action. The elucidation of the R-/S-MBTU mode of action would provide key information in the development of highly selective herbicides. The aim of this study was to elucidate the mode of action for wheat (Triticum aestivum L.) root growth inhibition by R-/S-MBTU using the cDNA SSH technique. Variation of mRNA levels of isolated clones was identified by quantitative real-time RT-PCR (qRT-PCR). These clones were related to disruption of amino acid biosynthesis, signal transduction, cellulose synthesis, and mitosis.
植物化学調節学会の論文
2008-10-06