41.サブトラクティブハイブリダイゼーション(SSH)法によるR-1-α-methylbenzyl-3-p-tolylurea処理におけるイネ根端発現遺伝子の探索(口頭発表)

概要

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The urea compound R-1-α-methylbenzyl-3-p-tolylurea (R-MBTU) shows very interesting property, R-MBTU inhibits root growth of Cyperus paddy weeds. It also inhibits root growth of Beckmannia syzigachne, a companion weed of wheat (Triticum aestivum L.), but not that of wheat. Thus, R-MBTU enantioselectively shows herbicidal activity, but there has been little information on the biochemical and molecular biological aspects for R-MBTU mode of action. The elucidation of the R-MBTU mode of action would provide key information in the development of highly selective herbicides. The aim of this study was to elucidate the mode of inhibited action of R-MBTU on rice (Oryza sativa L.) roots using the PCR-based SSH technique. Variations of mRNA levels of five genes was identified by quantitative real-time RT-PCR. These genes were related to disruption of amino acid biosynthesis, cellulose synthesis, mitosis, and repression of transcriptions of various genes.
植物化学調節学会の論文
2007-10-05