7. 超急速凍結保存法を用いた哺乳動物初期胚の凍結保存 : II.トランスジェニックマウス初期胚の凍結保存への応用(平成6年度第40回低温生物工学会研究報告)

概要

論文の詳細を見る
Transgenic mice have been widely utilized as a tool to evaluate the function of isolated gene in vivo. In order to develop the method of cryopreserving such genetically engineered mice, 2-cell embryos from transgenic mice expressing mouse OSF-1 (osteo-specific factor-1) cDNA were vitrified. Upon warming, 90.7% (107/118) were nomally recovered. When they were transferred to recipients, 45.8% developed to term. When the integrated pattern of transgene into the host genome and its expression level were compared between offspring derived from vitrified and those from unvitrified (control) transgenic embryos, there was neither significant alteration in the transgene integration pattern nor in the transgene expression level. These indicate that the vitrification-mediated cryopreservation does not only affect the expression level of transgene but also its integrated pattern in chromosomal DNA. By employing this technology, it will be possible to stock as many as embryos from transgenic lines without appreciable loss of transgene and its function.
低温生物工学会の論文
1994-12-27