6. 超急速凍結保存法を用いた哺乳動物初期胚の凍結保存 : I.マウス初期胚を用いての凍結保存法の開発(平成6年度第40回低温生物工学会研究報告)

概要

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Two-cell embryos were vitrified after 30-sec exposure to DP solution (2.75M DMSO and 2.75M propylene glycol in PB1) supplemented with 1.0M sucrose (DPS). They exhibited significantly higher in vitro survival rate (82%) than those vitrified with DP (44%). Similar high survival rate (81%) was obtained when they were vitrified with DP plus 0.16M raffinose (DPR). In vivo survival of 2-cell embryos vitrified after 30-sec exposure to DPS or DPR was both 49%, and there was no significant difference as compared with the unvitrified control group (60%). High in vitro survival rates (80-96%) were also obtained with embryos at 1-cell and morula stages vitrified after 30-sec exposure to DPS. In vivo survival rates of embryos at the 1-cell, morula and blastocyst stages were 56, 53 and 40%, respectively, when vitrified after 15-sec exposure to DPS. The results indicate that the vitrification procedure can be used for the cryopreservation of mouse preimplantation embryos at 1-cell, 2-cell and morula stages.
低温生物工学会の論文
1994-12-27