Enhanced differentiation of mesenchymal stem cells into NP-like cells via 3D co-culturing with mechanical stimulation(CELL AND TISSUE ENGINEERING)

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概要

• 論文の詳細を見る

• This study proposes a three-dimensional co-culturing system of mesenchymal stem cells (MSCs) and nucleus pulposus (NP) cells from New Zealand white male rabbits to differentiate MSCs into NP-like cells. The preferable ratio of MSCs to NP cells and the effects of mechanical stimulation were investigated without biochemical reagents. The preferable ratio was investigated without mechanical stimulation using five groups: Group I (MSC control); Group II (NP cell control); and Groups III, IV, and V, for which the ratios of NP cells to MSCs were 1:1, 1:2, and 2:1, respectively. During culture for 10 days without stimulation, the proliferation of MSCs did not increase after day 4. NP cells proliferated more when co-cultured as in Group V. However, the degree of differentiation of MSCs increased significantly in Group V. The differentiation of NP cells decreased gradually over time. When mechanical stimulation was applied to Groups I, II, and V, it contributed to the differentiation of MSCs into NP-like cells, as well as to that of NP cells, but did not contribute to the proliferation of either cell type. The contribution of mechanical stimulation to differentiation was also confirmed by RT-PCR.

• 社団法人日本生物工学会の論文

著者

• Shin Ji

  National Research Laboratory Of Cellular And Biomechanical Engineering Department Of Biomedical Engi

• Shin Ji

  National Res. Lab. Of Cellular And Biomechanical Engineering Dep. Of Biomedical Engineering Inje Uni

• Shin Jung-woog

  National Res. Lab. Of Cellular And Biomechanical Engineering Dep. Of Biomedical Engineering Inje Uni

• Shin Jung-woog

  National Research Laboratory Of Cellular And Biomechanical Engineering Department Of Biomedical Engi

• Kim Su-hyang

  National Research Laboratory Of Cellular And Biomechanical Engineering Department Of Biomedical Engi

• Kim Su-hyang

  National Res. Lab. Of Cellular And Biomechanical Engineering Dep. Of Biomedical Engineering Inje Uni

• Kim Dong

  National Institute of Scientific Investigation, Korea

• Heo Su-Jin

  National Research Laboratory of Cellular and Biomechanical Engineering, Department of Biomedical Eng

• Lee Shi

  National Research Laboratory of Cellular and Biomechanical Engineering, Department of Biomedical Eng

• Park Su

  Nano-Mechanical Systems Research Division, Korea Institute of Machinery and Materials

• Heo Su-jin

  National Research Laboratory Of Cellular And Biomechanical Engineering Department Of Biomedical Engi

• Lee Shi

  National Research Laboratory Of Cellular And Biomechanical Engineering Department Of Biomedical Engi

• Kim Dong

  National Research Laboratory Of Cellular And Biomechanical Engineering Department Of Biomedical Engi

• Park Su

  Nano-mechanical Systems Research Division Korea Institute Of Machinery And Materials

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