血清カリウム微量比色定量法

概要

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New colorimetric method for estimation of potassium in 0.1 ml. serum is described. In this method cerium ammonium nitrate and indigo carmine dye solution are prepared in the same way as blood calcium determination, which was reported in this journal. 1.5 ml. of water, 0.1 ml. of serum and 0.1 ml. of 16 per cent trichloracetic acid are mixed and from 1 ml. of clear supernatant potassium is precipitated as potassium sodium cobalti nitrite in a centrifuge tube. Precipitate is washed once with 5 ml. of 30 per cent alcohol and once more with 80 per cent alcohol. Clear colorless supernatant is drawn off by suction. 1 ml. of N/10 sodium hydroxyde is added and boiled in a water bath about 5 minutes. By this step potassium precipitate is dissolved and the solution is colored slight brown. 5 ml. of cerium ammonium nitrate (about N/1000) is added and centrifuge tube is filled to the mark of 10 ml. with water. 2 ml. of this mixture is pipetted in a test tube and 1 ml. of dye solution is added drop by drop during shaking. After adding of the 2 ml. of water blue color is measured photometrically. Calculation is as follows: K mg % = 19.5×E_2/E_1 E1: Reading of the extinction of standard solution (1 ml. of M/8000 potassium sulfate). E2: Reading of the extinction of 0.1 ml. serum.
千葉大学の論文
1954-03-28