血清ナトリウム微量比色定量法

概要

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In this institute microdetermination methods of blood components have been studied and reported during the past several years. In this report calorimetric method for the determination of sodium in 0.1 ml. serum is described. By this method iodometric pyroantimonate method ( Kramer-Tisdall) is improved and standardization of sodium thiosulfate is unnecessary. 1 ml. of water, 0.1 ml. of serum, 5 ml. of potassium pyroantimonate reagent, 3 ml. of 90 per cent alcohol (volume per cent) are mixed in a centrifuge tube and the latter is permitted to stand for about 60 minutes in the room temperature. Precipitated sodium pyroantimonate is washed twice with 5 ml. of 50 per cent alcohol and clear precipitate is dissolved with 5 ml. of concentrated hydrochloric acid. Then water is added to the mark of 10 ml. and mixed well. 2 ml. of this fluid and 1 ml. of 0.1 per cent potassium iodide, 5 ml. of benzene are mixed. After shaking vigorously red color solution is transferred to other test tube and small amount of sodium sulfate anhydrous is added. Extinction (10 mm.) of the red color solution (Filter S_<50>) is measured and the calculation of Na content of the serum is as follows: Na mg % = E×630
千葉大学の論文
1954-03-28