Simultaneous non-thermal saccharification of cassava pulp by multi-enzyme activity and ethanol fermentation by Candida tropicalis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
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Cassava pulp, a solid by-product from starch processing, is a promising and underused biomass that can be converted to biofuels and other value-added bio-products. In this study, an alternative cassava pulp saccharification process, which utilizes the multi-activity enzyme from Aspergillus niger BCC17849 and obviates the need for a pre-gelatinization step, was developed. The crude mufti-enzyme composed of non-starch polysaccharide hydrolyzing enzyme activities, including cellulase, pectinase and hemicellulase act cooperatively to release the trapped starch granules from the fibrous cell wall structure for subsequent saccharification by raw starch degrading activity. A high yield of fermentable sugars, equivalent to 716mg glucose and 67mg xylose/g of cassava pulp, was obtained after 48h incubation at 40℃ and pH5 using the multi-enzyme, which was greater than the yield obtained from the optimized combinations of the corresponding commercial enzymes. The multi-enzyme saccharification reaction can be performed simultaneously with the ethanol fermentation process using a thermotolerant yeast Candida tropicalis BCC7755. The combined process produced 14.3g/l ethanol from 4% (w/v) cassava pulp after 30h of fermentation. The productivity rate of 0.48g/l/h is equivalent to 93.7% of the theoretical yield based on total starch and cellulose, or 85.4% based on total fermentable sugars. The non-thermal enzymatic saccharification process described is more energy efficient and yields more fermentable sugar than the conventional enzymatic process. Furthermore, the process is applicable for production of various bio-products of economic importance.
- 公益社団法人日本生物工学会の論文
著者
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Champreda Verawat
Enzyme Technology Laboratory National Center For Genetic Engineering And Biotechnology
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Eurwilaichitr Lily
Enzyme Technology Laboratory National Center For Genetic Engineering And Biotechnology
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Rattanachomsri Ukrit
Enzyme Technology Laboratory National Center For Genetic Engineering And Biotechnology
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Tanapongpipat Sutipa
Enzyme Technology Laboratory Bioresources Technology Unit National Center For Genetic Engineering An
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Champreda Verawat
Enzyme Technology Laboratory Bioresources Technology Unit National Center For Genetic Engineering And Biotechnology
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