Identification and Characterization of Lipolytic Enzymes from a Peat-Swamp Forest Soil Metagenome

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概要

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• In this work, a metagenomic library was generated from peat-swamp forest soil obtained from Narathiwat Province, Thailand. From a fosmid library of approximately 15,000 clones, six independent clones were found to possess lipolytic activity at acidic pH. Analysis of pyrosequencing data revealed six ORFs, which exhibited 34–71% protein similarity to known lipases/esterases. A fosmid clone, designated LP8, which demonstrated the highest level of lipolytic activity under acidic conditions and demonstrated extracellular activity, was subsequently subcloned and sequenced. The full-length lipase/esterase gene, estPS2, was identified. Its deduced amino acid was closely related to a lipolytic enzyme of an uncultured bacterium, and contained the highly conserved motif of a hormone-sensitive family IV lipase. The EstPS2 enzyme exhibited highest activity toward p-nitrophenyl butyrate (C4) at 37 °C at pH 5, indicating that it was an esterase with activity and secretion characteristics suitable for commercial development.

• 社団法人 日本農芸化学会の論文
• 2010-09-23

著者

• Tanapongpipat Sutipa

  Enzyme Technology Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering

• Eurwilaichitr Lily

  Enzyme Technology Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering

• KANOKRATANA Pattanop

  Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology

• Thongaram Taksawan

  Enzyme Technology Laboratory Bioresources Technology Unit National Center For Genetic Engineering An

• BUNTERNGSOOK Benjarat

  Division of Biotechnology, School of Bioresources and Technology, King Mongkut's University of Techn

• UENGWETWANIT Tanaporn

  Enzyme Technology Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering

• RACHDAWONG Sansanalak

  Division of Biotechnology, School of Bioresources and Technology, King Mongkut's University of Techn

• VICHITSOONTHONKUL Taweerat

  Division of Biotechnology, School of Bioresources and Technology, King Mongkut's University of Techn

• Eurwilaichitr Lily

  Enzyme Technology Laboratory National Center For Genetic Engineering And Biotechnology

• Kanokratana Pattanop

  Enzyme Technology Laboratory National Center For Genetic Engineering And Biotechnology

• Tanapongpipat Sutipa

  Enzyme Technology Laboratory Bioresources Technology Unit National Center For Genetic Engineering An

• Rachdawong Sansanalak

  Division Of Biotechnology School Of Bioresources And Technology King Mongkut's University Of Te

• Uengwetwanit Tanaporn

  Enzyme Technology Laboratory Bioresources Technology Unit National Center For Genetic Engineering An

• Bunterngsook Benjarat

  Division Of Biotechnology School Of Bioresources And Technology King Mongkut's University Of Te

• Vichitsoonthonkul Taweerat

  Division Of Biotechnology School Of Bioresources And Technology King Mongkut's University Of Te

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