Estimation of dissolved carbon dioxide stripping in a large bioreactor using model medium(BIOCHEMICAL ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
Dissolved carbon dioxide (dCO_2) accumulation is one of the most serious problems in the scale-up of industrial cell culture. To predict the effects of dCO_2 stripping in different culture conditions and at different scales, we examined a method of estimation of dCO_2 stripping using a model medium. The operational parameters (e.g., sparging and agitation rate) and the size of the bioreactor (working volume: 80L, 500L, 2000L; aspect ratio: 1.0〜1.6) were varied, and the model medium was prepared by adjusting pH, density, viscosity, surface tension, and buffer conditions. dCO_2 stripping efficiency was evaluated using the index k_La^<CO_2>, which was defined in accordance with the volumetric oxygen transfer coefficient k_La. The model medium exhibited dCO_2 stripping behavior similar to real culture medium in all experimental conditions tested. It is expected that the use of the model medium to estimate dCO_2 stripping in real cultures will be valuable for determining the culture conditions in bioreactors in scale-up.
- 社団法人日本生物工学会の論文
著者
-
Matsunaga Naoki
Bio Process Research And Development Laboratories Kyowa Hakko Kirin Company Limited
-
DOBASHI Toshiaki
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University
-
Kano Kenjiro
Bio Process Research and Development Laboratories, Kyowa Hakko Kirin Company, Limited
-
Maki Yasuyuki
Department of Chemistry and Chemical Biology, Faculty of Engineering, Gunma University
-
Dobashi Toshiaki
Department Of Chemistry And Chemical Biology Faculty Of Engineering Gunma University
-
Kano Kenjiro
Bio Process Research And Development Laboratories Kyowa Hakko Kirin Company Limited
-
Maki Yasuyuki
Department Of Chemistry And Chemical Biology Faculty Of Engineering Gunma University
関連論文
- Coexistence Curves of Polystyrene in Cyclohexane near the Critical Double Point in Composition-Pressure Space : Condensed Matter: Structure, etc.
- Reentrant Three-Phase Equilibrium of Homologous Polystyrene Solution : Condensed Matter: Structure, etc.
- Shape of Three-Phase Coexistence Curven enar the Critical End Points for Four-Component Homologous Polystyrene Solution
- Enhanced Erythrocyte Sedimentation Rate and Upflow Layer in Inclined Rectangular Vessel
- Culture scale-up studies as seen from the viewpoint of oxygen supply and dissolved carbon dioxide stripping(BIOCHEMICAL ENGINEERING)
- Estimation of dissolved carbon dioxide stripping in a large bioreactor using model medium(BIOCHEMICAL ENGINEERING)
- Erythrocyte Aggregation Enhanced by Hydrostatic Pressure
- Deformability in Hardened and Normal Erythrocytes during Sedimentation Process
- Erythrocyte Sedimentation in Plasma Observed under Microscope
- Structural and Thermal Properties of Unpurified and Purified 12-Hydroxystearic Acid Solutions
- Fractal Analysis of Percolating Clusters with Chainlike Structures Formed in Polymer Matrix