PGE_2が歯根嚢胞および歯原性角化嚢胞由来細胞のHGF,IL-6およびPGE_2産生に及ぼす影響

概要

論文の詳細を見る
It is reported that dental cysts contain interleukin-1 (IL-1), which stimulates protaglandin secretion by fibrous capsule-derived fibroblasts. However, the mechanism of dental cyst expansion in alveolar bone remains unclear. The aim of this study was to simulate the autocrine effects of prostaglandin E_2 (PGE_2) on a growth factor for epithelial cells (hepatocyte growth factor, HGF), a bone resorbing cytokine (interleukin-6, IL-6), and PGE_2 production by human radicular cyst- and odontogenic keratocyst-derived fibroblast-like cells (RC cells and KC cells) in vitro. The confluent RC cells and KC cells and KC cells were exposed to 0.1 μmol/l of PGE_2 in serum-free DMEM for 0, 1, 3, 6, or 24 hours. The medium was harvested after 1, 3, 6, or 24 hours of each stimulation period. ELISA kits were employed to determine the amount of HGF, IL-6 and PGE_2 in the supernatant, respectively. PGE_2 significantly stimulated IL-6 production by RC cells and KC cells, and the effective stimulation time was 1 to 3 hours. PGE_2 stimulation also induced HGF production; however, the most effective stimulation time was 24 hours. Furthermore, PGE_2 stimulated PGE_2 production itself in the RC cells and KC cells, and every stimulation period was effective. These results show that PGE_2 stimulates HGF, IL-6 and PGE_2 production by RD cells and KC cells, suggesting that autocrine PGE_2 may induce radicular cyst and odontogenic keratocyst expansion once PGE_2 is produced in the cyst fibrous capsule.
特定非営利活動法人日本歯科保存学会の論文
2006-12-31

PGE_2が歯根嚢胞および歯原性角化嚢胞由来細胞のHGF,IL-6およびPGE_2産生に及ぼす影響

スポンサーリンク

概要

著者

関連論文

スポンサーリンク