Marker enrichment and construction of haplotype-specific BAC contigs for the polyembryony genomic region in Citrus

概要

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Polyembryony, in which zygotic and apomictic embryos develop concurrently in a seed, is widespread in Citrus. To obtain further genomic information about the previously characterized map position of the polyembryony locus, the relationship among linkage maps from 3 different hybrid populations was determined using common markers flanking the polyembryony locus. By mapping the polyembryony locus with cleaved amplified polymorphic sequence (CAPS) markers in a F180×'Harumi' population, the polyembryony locus was detected in a high-density marker region co-segregated to marker Mf0086. By screening a bacterial artificial chromosome (BAC) library with marker Mf0086, two contigs reflecting the polyembryony and monoembryony haplotype were constructed. The single nucleotide polymorphism (SNP) markers detected on these contigs were mapped on the nearest position to the polyembryony locus in linkage group 1 of 'Miyagawa wase' and 'Harumi'. The highly conserved marker lineage in the flanking regions of the polyembryony locus in these polyembryonic cultivars of different parentage suggested that the genomic region of the polyembryony locus might have a common structure in a wide range of Citrus cultivars.
日本育種学会の論文