Biological and Antibacterial Activities of the Natural Herb Houttuynia cordata Water Extract against the Intracellular Bacterial Pathogen Salmonella within the RAW 264.7 Macrophage(Microbiology)

スポンサーリンク

概要

• 論文の詳細を見る

• Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infection in animals and food-borne infection and typhoid fever in humans. Recently, the emergence of multidrug-resistant strains of Salmonella sp. has caused more serious problems in public health. The present study investigated the antibacterial effects of Houttuynia cordata water extract (HCWE) against murine salmonellosis. In RAW 264.7 cells, there was no detectable cytotoxic effect of HCWE at any concentration between 25 and 100μg/ml after 8-h incubation. The antibacterial activity of HCWE was then examined in a Salmonella enterica serovar (Salmonella typhimurium), and was found to increase in a dose-dependent manner at concentrations from 25 to 100μg/ml during 8-h incubation. HCWE also affected RAW 264.7 cells including morphologic change and bacterial uptake, but there was no significant difference in bacterial replication in RAW 264.7 cells. With HCWE alone, nitric oxide (NO) production by RAW 264.7 cells did not increase, but when RAW 264.7 cells were infected by S. typhimurium, with or without HCWE, NO production with HCWE was 2-fold higher than that without HCWE. Treatment with HCWE did not affect inducible NO synthase (iNOS) mRNA expression by RAW 264.7 cells, but when RAW 264.7 cells with HCWE were infected by S. typhimurium, iNOS mRNA expression was increased during 8-h incubation. Furthermore, HCWE showed virulence reduction effects in S. typhimurium-infected BALB/c mice. After a lethal dose of S. typhimurium, the mortality rate in the HCWE untreated group was 100% at 7 d, but the HCWE 25, 50, and 100μg/ml groups survived until 11, 17, and 23 d, respectively. These data suggest that HCWE is stable and beneficial in the treatment of bacterial infection including intracellularly replicating pathogens and may solve antimicrobial misuse and overuse.

• 社団法人日本薬学会の論文
• 2008-11-01

著者

• Lee Hu

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• LEE Jin

  Institute of Agriculture and Life Science, Gyeongsang National University

• KIM Dong

  Institute of Agriculture and Life Science, Gyeongsang National University

• LIM Jeong

  Institute of Agriculture and Life Science, Gyeongsang National University

• KIM Suk

  Institute of Agriculture and Life Science, Gyeongsang National University

• Won Chung

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• Lim Jeong

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• Kim Gon

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• Rhee Man

  韓国

• Rhee Man

  Department Of Physiology College Of Veterinary Medicine Kyungpook National University

• Han Dae

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• JUNG Won

  Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University

• LEE Whi

  Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University

• MIN Won

  Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University

• Jung Won

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• Lee Whi

  Department Of Veterinary Medicine College Of Veterinary Medicine Kyungpook National University

• Kim Suk

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University:institute

• Min Won

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• Kim Dong

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• LEE Hu

  Institute of Agriculture and Life Science, Gyeongsang National University

関連論文

• Characterization and Identification of Erysipelothrix rhusiopathiae Isolated from Unnatural Host, a Cat, with a Clinical Manifestation of Depression
• 固相蛍光免疫測定法によるヒラメ(Paralichthys olivaceus)組織中の残留オキシテトラサイクリンとテトラサイクリンの測定,魚組織中のテトラサイクリンスクリーニング試験法(公衆衛生学)
• Biological and Antibacterial Activities of the Natural Herb Houttuynia cordata Water Extract against the Intracellular Bacterial Pathogen Salmonella within the RAW 264.7 Macrophage(Microbiology)
• アヒルに皮下投与後のセフチオフルの血漿と組織における分布(公衆衛生学)
• 部位特異性を示すトロンビンの星状膠細胞星状化に対する影響(解剖学)
• Bacteriology: Characterization and identification of Erysipelothrix rhusiopathiae isolated from an unnatural host, a cat, with a clinical manifestation of depression
• In Vitro Immunoregulatory Effects of Korean Mistletoe Lectin on Functional Activation of Monocytic and Macrophage-Like Cells(Pharmacology)
• Application of immunoproteomics in developing a Streptococcus iniae vaccine for olive flounder (Paralichthys olivaceus)
• Prevalence of Lawsonia intracellularis, Salmonella spp. and Eimeria spp. in Healthy and Diarrheic Pet Rabbits

スポンサーリンク