Comparative Effect of Overexpressed phaJ and fabG Genes Supplementing (R)-3-Hydroxyalkanoate Monomer Units on Biosynthesis of mcl-Polyhydroxyalkanoate in Pseudomonas putida KCTC1639(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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phaJ and fabG genes encoding (R)-specific 2-enoyl-CoA hydratase and 3-ketoacyl-acyl carrier protein reductase, respectively, were cloned from Pseudomonas putida KCTC1639 and amplified in the parent strain to achieve oversupplementation of (R)-3-hydroxyalkanoate monomer units for the biosynthesis of medium-chain-length polyhydroxyalkanoate (mcl-PHA). The comparative effect of the overexpressed fabG and phaJ genes in P. putida KCTC1639 on the biosynthesis of mcl-PHA and the cell growth were elucidated. Overexpression of phaJ enhanced the biosynthesis of mcl-PHA, increasing its content and concentration from 18% to 27% and 0.38 to 0.51 g/l, respectively. Conversely, fabG overexpression tended to depress the biosynthesis of mcl-PHA, possibly due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA.
- 社団法人日本生物工学会の論文
- 2008-07-25
著者
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LEE KWANG-WOO
Department of Internal Medicine, College of Medicine, The Catholic University of Korea
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Lee Kwang-woo
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
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Jung Young-mi
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
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Lee Yong-hyun
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
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Lee Yong-hyun
Department Of Genetic Engineering College Of Natural Science Kyoungpook National University
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Vo Minh
Department of Genetic Engineering, College of Natural Sciences Kyungpook National University
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Vo Minh
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
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Lee Yong-hyun
Department Ceramic Engineering, Hanyang University, Seoul 133-791, Korea
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