Role of tktA Gene in Pentose Phosphate Pathway on Odd-Ball Biosynthesis of Poly-β-hydroxybutyrate in Transformant Escherichia coli Harboring phbCAB Operon(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
A cloned tktA gene encoding transketolase (TK), the most critical enzyme in the nonoxidative pentose phosphate (PP) pathway, was reinforced into the parent Escherichia coli for metabolic flux control of carbohydrate. It was also transformed into the transformant E. coli harboring phbCAB operon for the odd-ball biosynthesis of PHB. The biosynthesis of PHB significantly increased up to 1.7-fold after the cotransformation of the phbCAB operon and tktA gene, due to the active supplementation of the precursor molecules, acetyl-CoA and NADPH.
- 2004-09-25
著者
-
Jung Y‐m
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
-
Jung Young-mi
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
-
Lee Y‐h
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
-
Shin Hyun-dong
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
-
Lee Yong-hyun
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
-
Lee Yong-hyun
Department Of Genetic Engineering College Of Natural Science Kyoungpook National University
-
LEE JIN-NAM
Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University
-
Lee Jin-nam
Department Of Genetic Engineering College Of Natural Sciences Kyungpook National University
-
Lee Yong-hyun
Department Ceramic Engineering, Hanyang University, Seoul 133-791, Korea
-
Shin Hyun-Dong
Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University
関連論文
- Role of tktA Gene in Pentose Phosphate Pathway on Odd-Ball Biosynthesis of Poly-β-hydroxybutyrate in Transformant Escherichia coli Harboring phbCAB Operon(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Utilization of Oxidative Pressure for Enhanced Production of Poly-β-Hydroxybutyrate and Poly(3-Hydroxybutyrate-3-Hydroxyvalerate) in Ralstonia eutropha
- 606 CHARACTERIZATION OF CYCLODEXTRIN GLUCANOTRANSFERASE RROM Bacillus firmus var. alkalophilus BY CHEMICAL MODIFICATION
- Comparative Effect of Overexpressed phaJ and fabG Genes Supplementing (R)-3-Hydroxyalkanoate Monomer Units on Biosynthesis of mcl-Polyhydroxyalkanoate in Pseudomonas putida KCTC1639(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Regulating the Molar Fraction of 4-Hydroxybutyrate in Poly(3-Hydroxybutyrate-4-hydroxybutyrate) Biosynthesis by Ralstonia eutropha Using Propionate as a Stimulator
- Modulation of talA Gene in Pentose Phosphate Pathway for Overproduction of Poly-β-Hydroxybutyrate in Transformant Escherichia coli Harboring phbCAB Operon(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- 2D17-2 Enterobacter sp. BL-2 Excreting Microbial Polyglucosamine Biopolymer and Its Morphological Variation under Acetate-Mediated pH Environment
- Metabolic Characteristics of Isocitrate Dehydrogenase Leaky Mutant of Alcaligenes eutrophus and Its Utilization for Poly-β-Hydroxybutyrate Production
- 1068 Metabolic Engineering of Alcaligenes eutrophus for Investigation of Regulatory Mechanism of PHB and P(3HB-3HV) Biosynthesis
- 345 Metabolic Characteristics of Isocitrate Dehydrogenase Leaky Mutant of Alcaligenes eutrophus and Its Utilization for Poly-β-hydroxybutyrate Production
- Amplification of the NADPH-Related Genes zwf and gnd for the Oddball Biosynthesis of PHB in an E. coli Transformant Harboring a Cloned phbCAB Operon
- Piezoelectric Properties and Densification Based on Control of Volatile Mass of Potassium and Sodium in (K0.5Na0.5)NbO3 Ceramics