Modulation of talA Gene in Pentose Phosphate Pathway for Overproduction of Poly-β-Hydroxybutyrate in Transformant Escherichia coli Harboring phbCAB Operon(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

概要

論文の詳細を見る
The talA gene encoding transaldolase, the key enzyme in the nonoxidative pentose phosphate pathway, was amplified in a transformant Escherichia coli harboring the phbCAB operon to shift the metabolic flux of the hexose mono-phosphate shunt to the odd-ball biosynthesis pathway for poly-β-hydroxybutyrate overproduction. The PHB content in the transformant E. coli coharboring the phbCAB operon and talA gene increased from 28.2% to 52.3%, and the retarded cell growth was overcome. This increase seems to be mainly due to the concomitant supplies of the intermediates NADPH and acetyl-CoA, which are from the activated pentose phosphate pathway through the modulation of the talA gene and from the Embden-Meyerhof pathway.
社団法人日本生物工学会の論文
2006-09-25