Production of Glycerol from Glucose by Coexpressing Glycerol-3-Phosphate Dehydrogenase and Glycerol-3-Phosphatase in Klebsiella pneumoniae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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As a valuable chemical, 1,3-propanediol(1,3-PD) could be biosynthesized by glycerol fermentation. However, no natural microorganisms that could directly convert glucose into 1,3-PD have been found so far. In this work, genes coding for two enzymes, glycerol-3-phosphate dehydrogenase(GPD, EC 1.1.1.8) and glycerol-3-phosphatase(GPP, EC 3.1.3.21), which were responsible for glycerol production, were organized into the plasmid pUC18K under control of the respective lac promoters. Two recombinant proteins were expressed successfully in wild-type Klebsiellapneumoniae. A glycerol concentration of 6.8 gl^<-1> was obtained in flask culture. When glucose was exhausted, dihydroxyacetone was added and medium pH was adjusted to 7.0, and then a 1,3-PD concentration of 0.58 gl^<-1> was achieved with engineered K. pneumoniae from glucose.
- 社団法人日本生物工学会の論文
- 2008-05-25
著者
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Zhang Jianguo
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Zhao Li
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Zheng Yu
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Zhang Haiyi
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Ma Xingyuan
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Wei Dongzhi
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
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Zheng Yu
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Ma Xingyuan
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Zhang Haiyi
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
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Wei Dongzhi
State Key Laboratory Of Bioreactor Engineering East China University Of Science And Technology
関連論文
- Production of Glycerol from Glucose by Coexpressing Glycerol-3-Phosphate Dehydrogenase and Glycerol-3-Phosphatase in Klebsiella pneumoniae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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