Identification of Aminotadalafil and its Stereoisomers Contained in Health Foods Using Chiral Liquid Chromatography-Mass Spectrometry
スポンサーリンク
概要
- 論文の詳細を見る
Using chiral liquid chromatography-mass spectrometry (LC/MS), a simple and rapid identification test was developed for aminotadalafil [(6R,12aR)-2-amino-6-(1,3-benzodioxol-5-yl)-2,3,6,7,12,12a-hexahydropyrazino[l',2' : 1,6]pyrido[3,4-b]indole-l,4-dione; RR-ATDF] and its stereoisomers contained in health foods, e.g. herbal products. A sample solution was prepared using methanol extraction. Analysis was performed on a chiral column with the mixture of 0.1% formic acid/acetonitrile (7: 3) as mobile phase at 30℃. Each resolution value of four stereoisomers of ATDF was greater than 1.3. A mass spectrometer was used as a detector to enhance specificity by excluding the effects of general components derived from the sample. The four individual stereoisomers of ATDF in the health foods were identified based on their respective retention times. Results showed that structural conversions of RR-ATDF into (6R,12aS)-2-amino-6-(1,3-benzodioxol-5-yl)-2,3,6,7,12,12a-hexahydropyrazino[1',2' : 1,6]pyrido[3,4-b]indole-1,4-dione (RS-ATDF) and (6S,12aS)-2-amino-6-(1,3-benzodioxol-5-yl)-2,3,6,7, 12, 12a-hexahydropyrazino[1',2' : 1,6]pyrido[3,4-b]indole-1,4-dione (SS-ATDF) into (6S,12aR)-2-amino-6-(1,3-benzodioxol-5-yl)-2,3,6,7,12,12a-hexahydropyrazino[1',2' : 1,6]pyrido[3,4-b]indole-1,4-dione (SR-ATDF) occurred under strongly basic conditions, which indicates that such conditions must be avoided during sample preparation. Using this method, RR-ATDF and SR-ATDF were detected in a health food; this is the first report describing that ATDF diastereomers are present in health foods.
- 2008-06-01
著者
-
MIZUNO KUMIKO
Department of Central Clinical Laboratory, Nagoya City University Hospital
-
Mizuno Kumiko
Department Of Agricultural Chemistry Faculty Of Agriculture Yamaguchi University
-
Mizuno Kumiko
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
-
Yamamoto Masatoshi
Department Of Applied Biology Kyoto Institute Of Technology
-
Yamamoto Masatoshi
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
-
KUROMI Koichi
Department of Medical Biochemistry, University of Shizuoka School of Pharmaceutical Sciences
-
SUZUKI Nobuyuki
Department of Joint Disease and Rheumatism, Nippon Medical School
-
Owada Kazuhiro
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
-
Kuromi Koichi
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
-
Kurita Hiroyuki
Department of Drug and Food Science, Shizuoka Institute of Environment and Hygiene
-
Ueno Chie
Department of Drug and Food Science, Shizuoka Institute of Environment and Hygiene
-
Kamimura Mitsuko
Department of Drug and Food Science, Shizuoka Institute of Environment and Hygiene
-
Fujiwara Atsuko
Department of Drug and Food Science, Shizuoka Institute of Environment and Hygiene
-
Ogo Naohisa
Department of Drug and Food Science, Shizuoka Institute of Environment and Hygiene
-
Ueno Chie
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
-
Ogo Naohisa
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
-
Kurita Hiroyuki
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
-
Fujiwara Atsuko
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
-
Kamimura Mitsuko
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
-
Suzuki Nobuyuki
Department Of Drug And Food Science Shizuoka Institute Of Environment And Hygiene
関連論文
- Cardiac Vagal Activation by Adrenocorticotropic Hormone Treatment in Infants with West Syndrome
- Angioscopic Study of Silent Plaque Disruption in Nonischemic Related Coronary Artery in Patients With Stable Ischemic Heart Disease
- Transgemeration Carcinogenesis in the Progeny of Irradiated Male Mice
- Isolation of a Novel Peptide Homing to Tumor-Derived Neovasculature That Suppresses Tumor Growth
- Selective accumulation of CCR5^+ T lymphocytes into inflamed joines of rheumatiod arthritis
- Further genetic studies on the Katsunuma population of Drosophila melanogaster
- Ascorbate Oxidase in Cucumber Calli and Enhancement of Enzyme Activity by Copper Sulfate
- Purification and Properties of Ascorbate Oxidase from Cucumber Fruit
- Identification of Aminotadalafil and its Stereoisomers Contained in Health Foods Using Chiral Liquid Chromatography-Mass Spectrometry
- Experimental Research of Caries Prevention by Er,Cr:YSGG Laser
- Cancer Chemotherapy by Liposomal 6-[[2-(Dimethylamino)ethyl]amino]-3-hydroxy-7H-indeno [2,1-c] quinolin-7-one Dihydrochioride (TAS-103), a Novel Anti-cancer Agent(Miscellaneous)
- The Meaning of Readmission in Morita Therapy : From the treatment experience of morbid fear of vomiting
- A Structural Analysis of the Regularly Arranged Porin on the Outer Membrane of Campylobacter jejuni Based on Correlation Averaging
- Callus Induction by Optically Active Auxin, R-(+)-2-(2-Naphthoxy)propionic Acid
- IgG rheumatoid factor in early rheumatoid arthritis as a predictor of radiological changes and disease activity
- PB39 SYNTHESIS OF CHIRAL CYCLOPENTANOIDS BY ASYMMETRIC ENE REACTIONS VIA CHIRAL ALLYLIC SULFINATE-SULFONE REARRANGEMENTS