84(P71) 高等生物DNAポリメラーゼ阻害物質の探索および構造決定(ポスター発表の部)

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We have been studying structure and function of eukaryotic DNA polymerases, which are divided into at least 6 classes designated as α, β, γ, δ, ε and ζ. In the process of our investigations, the need for an inhibitor of each of the DNA polymerases has arisen. The roles of the DNA polymerases in vivo are still mostly obscure, and for the elucidation of the precise roles of each DNA polymerase, the use of the appropriate inhibitors would be quite useful. We therefore have established an assay method to detect DNA polymerase inhibitors, and we have used it to screen the extracts of many organisms for the inhibitors. In the screening, an important aspect was the type of natural product as a source of inhibitors. Not only several fungi, mushrooms and higher plants were found to produce such inhibitors, but also some algae were indicated to produce them. We found inhibitors from a basidiomycete, Fomitella fraxinea, well-known fatty acids and novel triterpenoids (fomitellic acids) (Fig. 1). We also found from a basidiomycete, Ganoderma lucidum, an ergosterol peroxide (Fig. 2), incisterols (Fig. 3) and two cerebrosides which were called the fruiting body-inducing substances (Fig. 4). Sulfolipids (Sulfoquinovosyl diacylglycerol, SQDG) (Fig. 5), which is the strongest DNA polymerase inhibitors, were found in a fern, Athyrium niponicum, and sea alga, Gigartina tenella. The fern compounds were isolated and identified as previously identified sulfolipid compounds from a cyanobacteria which are AIDS-antiviral agents.
天然有機化合物討論会の論文
1998-08-31

84(P71) 高等生物DNAポリメラーゼ阻害物質の探索および構造決定(ポスター発表の部)

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