Response to GA and Variation of the Culm Length in Doubled Haploid Lines of Wheat(Crop Physiology & Ecology)
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概要
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An F_1 hybrid with the semi dwarf genes, Rhtl and Rht2, was crossed with Hordeum bulbosum and maize (teosinte) (bulbosum method and maize method) to produce doubled haploid (DH) lines, and 102 seedlings of each DH line produced by the two methods were treated with GA. The sensitivity to GA was shown by the GA index (GAI) which is (length of the first leaf sheath (LS) in GA-treated seedlings / length of LS in GA-untreated seedlings) × 100. The scatter diagram of GAI plotted against culm length was divided into three groups, low, medium and high GAI groups. The segregation ratio of these gametophytic phenotypes was close to the expected ratio; 1:2:1=KhtlRht2: (Rht1rhtZ+rht1RhtZ):rht1rht2, in both DH lines produced by the two methods. The frequency distribution of culm length in DH_3, DH_4, DH_5 and DH_6 populations from DH produced by the bulbosum method showed two peaks, though that in F_3, F_4, F_5 and F_6 populations from the same F_1 hybrid (non-selective population) showed a continual normal distribution. The ratio of individuals with a shorter culm length to those with a longer culm length in DH lines was close to the expected ratio; 1:3 [Rht1Rht2: (Rht1rht2+rht1Rht2+rht1rht2)]. The frequency distribution of culm length in F_6 was significantly different from that in F_3-F_5 populations, indicating an unintentional selection during the generation of the selfed hybrid, but the frequency of short culm relative to long culm individuals was lower in F_3-F_6 populations than in DH lines. These findings suggest the usefulness of selecting the medium culm-length lines in the DH lines produced by the bulbosum or maize method in addition to examining the GA sensitivity (GAI).
- 日本作物学会の論文
著者
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Ushiyama Tomohiko
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology
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Ushiyama Tomohiko
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology:(prese
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Yoshida Tomohiko
Facurty of Agriculture, Utsunomiya University
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Ushiyama Tomohiko
United Graduate School Of Agricultural Sci. Tokyo Univ. Of Agriculture And Technol.
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Yoshida Tomohiko
Facurty Of Agriculture Utsunomiya University
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