Effects of Various Phytohormones on Haploid Wheat Production in Wheat x Maize Crosses(Crop Physiology and Ecology)

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概要

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• The effects of phytohormones on the production of haploid wheat were examined in the intergeneric crosses between Japanese wheat cultivar (Triticum aestivum cv. Zenkojikomugi) and maize (Zea mays cv. Pioneer P80 Lisa). The detached wheat spikes pollinated with maize were cultured in a solution containing sucrose and sulfurous acid supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), indole-acetic acid, naphthalene acetic acid, kinetin or 6-benzylaminopurine at 0, 0.1, 1, 10, 100 or 1000mg L^<-1>. Haploid embryos obtained were cultured on agarose-solidified B5 medium. The frequency of plant regeneration was significantly affected only by the treatment with 100mg L^<-1> 2,4-D. Thus, the detached spikes were cultured in the medium containing 2,4-D at 0, 25, 50, 75, 100, 125, 150, 175, 200 or 400mg L^<-1>. The treatments with 50mg L^<-1> 2,4-D increased the embryo size, but the treatments with above 75mg L^<-1> 2,4-D inhibited the development of the embryo. The percentage of florets developing into haploid plants was increased by the treatment with 100mg L^<-1> 2,4-D. Therefore, the concentration of 2,4-D in the spikes treated with 2,4-D at 50, 100 and 150mg L^<-1> were measured by gas chromatography. The concentration of 2,4-D in the seed was increased to 9.24 ppm by the treatment with 100mg L^<-1> 2,4-D, a further increase of 2,4-D concentration in the medium having no effect. On the other hand, the concentration of 2,4-D in the glumes and rachis increased up to 12.72 and 41.55 ppm by the treatment with 100 and 150mg L^<-1> 2,4-D, respectively. The treatments with 2,4-D at a concentration higher than 100mg L^<-1> inhibited embryo development. The present results suggested that 2,4-D at the concentrations from 50 to 100mg L^<-1> would be optimum for haploid wheat production using maize.

• 日本作物学会の論文

著者

• Yoshida Tomohiko

  Fac. Of Agriculture Utsunomiya Univ.

• Kuwabara Tatsuo

  National Agricultural Research Center For Hokkaido Region

• Ushiyama Tomohiko

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology

• Yoshida Tomohiko

  Facurty of Agriculture, Utsunomiya University

• Ushiyama Tomohiko

  United Graduate School Of Agricultural Sci. Tokyo Univ. Of Agriculture And Technol.

• Yoshida Tomohiko

  Facurty Of Agriculture Utsunomiya University

• Yoshida Tomohiko

  Faculty of Agriculture, Utsunomiya University

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• Effect of 2,4-Dichlorophenoxyacetic Acid on the Efficiency of Wheat Haploid Production by the Hordeum bulbosum Method(Crop Physiology and Ecology)
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