Effect of 2,4-Dichlorophenoxyacetic Acid on the Efficiency of Wheat Haploid Production by the Hordeum bulbosum Method(Crop Physiology and Ecology)
スポンサーリンク
概要
- 論文の詳細を見る
Intergeneric crosses between Japanese wheat cultivars (Triticum aestivum L. cv. Nishikazekomugi and Zenkojikomugi) and a tetraploid wild barley, Hordeum bulbosum L. were used to examine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on the formation of wheat haploid embryo and its development into plantlets. The detached wheat spikes with florets pollinated with H. bulbosum were cultured for 14 days in a sucrose and sulfurous acid solution to which 2,4-D was added at the concentrations of 0, 25, 50, 75, 100, 125, 150, and 175mg L^<-1>. The percentages of florets with seeds set and with embryos formed were increased by increasing the concentration of 2,4-D up to 100mg L^<-1>. Fourteen days after pollination, embryos (haploid) were isolated from the seed and cultured on agarose-solidified B5 medium. Embryo size tended to decrease as the concentration of 2,4-D increased, but the larger embryos tended to have higher ability to develop into haploid plants. The percentage of florets from which haploid plantlets were developed by embryo culture was slightly increased by the treatment of the spikes with 25-100mg L^<-1> 2,4-D, but significantly reduced by 125-175mg L^<-1> 2,4-D. It is suggested that treatment with 2,4-D at 25 100mg L^<-1> would be effective for haploid wheat production by of H. bulbosum method.
- 日本作物学会の論文
著者
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Yoshida Tomohiko
Fac. Of Agriculture Utsunomiya Univ.
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Kuwabara Tatsuo
National Agricultural Research Center For Hokkaido Region
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Ushiyama Tomohiko
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology
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Yoshida Tomohiko
Facurty of Agriculture, Utsunomiya University
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Ushiyama Tomohiko
United Graduate School Of Agricultural Sci. Tokyo Univ. Of Agriculture And Technol.
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Yoshida Tomohiko
Facurty Of Agriculture Utsunomiya University
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Yoshida Tomohiko
Faculty of Agriculture, Utsunomiya University
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