Role of Heat Shock Protein 70 in Induction of Stress Fiber Formation in Rat Arterial Endothelial Cells in Response to Stretch Stress
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概要
- 論文の詳細を見る
We investigated the mechanism by which endothelial cells (ECs) resist various forms of physical stress using an experimental system consisting of rat arterial EC sheets. Formation of actin stress fibers (SFs) and expression of endothelial heat-shock stress proteins (HSPs) in response to mechanical stretch stress were assessed by immunofluorescence microscopy. Stretch stimulation increased expression of HSPs 25 and 70, but not that of HSP 90. Treatment with SB203580, a p38 MAP kinase inhibitor that acts upstream of the HSP 25 activation cascade, or with geldanamycin, an inhibitor of HSP 90, had no effect on the SF formation response to mechanical stretch stress. In contrast, treatment with quercetin, an HSP 70 inhibitor, inhibited both upregulation of endothelial HSP 70 and formation of SFs in response to tensile stress. In addition, treatment of stretched ECs with cytochalasin D, which disrupts SF formation, did not adversely affect stretch-induced upregulation of endothelial HSP 70. Our data suggest that endothelial HSP 70 plays an important role in inducing SF formation in response to tensile stress.
- 日本組織細胞化学会の論文
著者
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TAKEMASA Tohru
Institute of Health and Sport Sciences, University of Tsukuba
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Fu Song-bin
Laboratory Of Medical Genetics Harbin Medical University
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Yamashita Kazuo
Department Of Applied Chemistry Graduate School Of Engineering Hiroshima University
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Takemasa Tohru
Institute Of Health & Sports Sciences University Of Tsukuba
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SUGIMOTO Keiji
Department of Neurosurgery, Shimane University School of Medicine
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Sugimoto Keiji
Department Of Molecular Anatomy Nippon Medical School
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Sugimoto Keiji
Department Of Anatomy Nippon Medical School
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Luo Shan-Shun
Department of Molecular Anatomy, Nippon Medical School
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Fujii Sachiko
Department of Molecular Anatomy, Nippon Medical School
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Luo Shan-shun
Department Of Molecular Anatomy Nippon Medical School:department Of Internal Medicine Harbin Medical
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Fujii Sachiko
Department Of Molecular Anatomy Nippon Medical School
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Yamashita Kazuo
Department Of Molecular Anatomy Nippon Medical School
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Yamashita Kazuo
Department Of Anatomy Nagoya University School Of Medicine
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Takemasa Tohru
Institute of Health and Sports Sciences, University of Tsukuba
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