Identification of Hop Polyphenolic Components Which Inhibit Prostaglandin E_2 Production by Gingival Epithelial Cells Stimulated with Periodontal Pathogen(Pharmacognosy)
スポンサーリンク
概要
- 論文の詳細を見る
Chronic marginal periodontitis is a destructive inflammatory disease caused by an imbalance between bacterial virulence and host defense ability, resulting in eventual tooth exfoliation. Porphyromonas gingivalis, a major periodontal pathogen, triggers a series of cellular inflammatory responses including the production of prostaglandin E_2 (PGE_2), which causes periodontal destruction; thus, anti-inflammatory reagents are considered beneficial for periodontal therapy. In the present study, we examined whether hop- and apple-derived polyphe-nols (HBP and ACT, respectively) inhibit PGE_2 production by human gingival epithelial (HGE) cells stimulated with P. gingivalis components. HGE cells were stimulated with P. gingivalis membrane vesicles, and the effects of HBP, ACT and epigallocatechin gallate (EGCg) on PGE_2 production by HGE cells were evaluated using an en-zyme-linked immunosorbent assay. HBP and EGCg significantly inhibited PGE_2 production, whereas ACT did not. By further fractionation steps of HBP to identify the effective components, 3 components of HBP, 2-[(2-methylpropanoyl)-phloroglucinol]1-O-β-D-glucopyranoside (MPPG), quercetin 3-O-β-D-glucopyranoside (isoquercitrin), and kaempferol 3-O-β-glucopyranoside (astragalin), were found to be elements which significantly inhibited cellular PGE_2 production. These results suggest that HBP is a potent inhibitor of cellular PGE_2 production induced by P. gingivalis, and HBP may be useful for the prevention and attenuation of periodontitis.
- 2008-03-01
著者
-
Honma Daiki
Fundamental Research Laboratory Asahi Breweries Ltd.
-
INABA Hiroaki
Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry
-
TAGASHIRA Motoyuki
Fundamental Research Laboratory, Asahi Breweries Ltd.
-
KANDA Tomomasa
Fundamental Research Laboratory, Asahi Breweries Ltd.
-
KOU Yurong
Department of Oral Medicine, School of Stomatology, China Medical University
-
OHTAKE Yasuyuki
Fundamental Research Laboratory, Asahi Breweries Ltd.
-
AMANO Atsuo
Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry
-
Kou Yurong
Department Of Oral Medicine School Of Stomatology China Medical University
-
Amano Atsuo
Department Of Oral Frontier Biology Osaka University Graduate School Of Dentistry
-
Inaba Hiroaki
Department Of Oral Frontier Biology Osaka University Graduate School Of Dentistry
-
Kanda Tomomasa
Fundamental Research Laboratory Asahi Breweries Ltd.
-
Ohtake Yasuyuki
Fundamental Research Laboratory Asahi Breweries Ltd.
-
Tagashira Motoyuki
Fundamental Research Laboratory Asahi Breweries Ltd.
-
Kanda Tomomasa
Fundamental Research Laboratory Asahi Breweries Limited
-
Amano Atsuo
Department Of Oral Frontier Biology Graduate School Of Dentistry Osaka University
-
Inaba Hiroaki
Department Of Oral Frontier Biology Graduate School Of Dentistry Osaka University
-
Homma Daiki
Fundamental Research Laboratory, Asahi Breweries Ltd.
関連論文
- Identification of Hop Polyphenolic Components Which Inhibit Prostaglandin E_2 Production by Gingival Epithelial Cells Stimulated with Periodontal Pathogen(Pharmacognosy)
- Etiologic factors of early-onset periodontal disease in Down syndrome
- Gingival Overgrowth in Rats Orally Treated with Large Dose of Phenytoin
- Clinical evaluation of orofacial regulation therapy for Down syndrome children using Castillo-Morales palatal plate
- Inhibitory Effect of Polyphenol-Enriched Apple Extracts on Mast Cell Degranulation in Vitro Targeting the Binding between IgE and FcεRI
- Gene Expression Analysis of the Anti-obesity Effect by Apple Polyphenols in Rats Fed a High Fat Diet or a Normal Diet
- Roles of Oral Bacteria in Cardiovascular Diseases — From Molecular Mechanisms to Clinical Cases: Implication of Periodontal Diseases in Development of Systemic Diseases
- Effects of Dietary Procyanidins and Tea Polyphenols on Adipose Tissue Mass and Fatty Acid Metabolism in Rats on a High Fat Diet
- Apple Polyphenols Influence Cholesterol Metabolism in Healthy Subjects with Relatively High Body Mass Index
- Serum Cholesterol-Lowering Effect of Apple Polyphenols in Healthy Subjects
- Autophagy in Innate Immunity against Intracellular Bacteria
- Apple Procyanidins Induced Vascular Relaxation in Isolated Rat Aorta through NO/cGMP Pathway in Combination with Hyperpolarization by Multiple K^+ Channel Activations
- The Oral Microbiology Research of Shigeyuki Hamada in the Pre-genomic Era
- Consideration on English education at Osaka University Graduate School of Dentistry
- Roles of Oral Bacteria in Cardiovascular Diseases-From Molecular Mechanisms to Clinical Cases : Implication of Periodontal Diseases in Development of Systemic Diseases