Efficient Plant in vitro Regeneration of Pinelliae Cordatae Breit(Crop Physiology & Ecology)

概要

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Pinelliae cordatae, one species of Pinellia genus, is a kind of rare wild plant growing in the dank around rocks of hill or cliff. It also has been a traditional Chinese medical herb applied on treating snake biting bite, stomachache and lumbago for years. Moreover, it may be used as a good ornamental plant for its diminutive stature, nice leaves and inflorescence. With the development of this traditional Chinese medicine exploiting nowadays, P. cordatae is more and more in demand. The wild sources of P. cordatae are in danger of being exhausted due to overcollection, inadequate propagation, and rigorous growing environment limitation. It is, therefore, important to find an effective way of propagation of P. cordatae to protect wild resource and solve the shortage problems. Plant tissue culture and somatic regeneration has been demonstrated to be successful in achieving rapid propagation of various plant species (Gamborg, 2002). To date, however, there have been no reports on the plant culture in vitro of P. cordatae. In this paper, we report an efficient plant regeneration program of P. cordatae. Factors such as explant type, medium type and growth regulator combination were evaluated for determination of effective.
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